FIGURE

Figure 1

ID

ZDB-FIG-230228-423

Publication

Sellaththurai et al., 2023 - CRISPR/Cas9-Induced Knockout of Sting Increases Susceptibility of Zebrafish to Bacterial Infection

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

Generation of sting^(−/−) zebrafish. (A) Schematic illustration of organization of zebrafish sting. The white and black boxes represent untranslated regions and open reading frames, respectively. The blue arrowhead indicates the single guide RNA (sgRNA) target site. The PAM and the sgRNA target sequences are indicated in red and blue letters, respectively. (B) The obtained mutation in sting^(−/−) fish using CRISPR/Cas9 gene editing. Deleted nucleotides in the sting^(−/−) genome are indicated with hyphens. (C) Amino acid prediction of Sting in the sting^{(−/− )} and WT zebrafish genomes. The altered amino acids are represented with red letters. (D) Confirmation of mutation via RT–PCR using sting^(−/−) mutation-specific primers in 7 dpf larvae of sting^(−/−) and WT zebrafish.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomolecules