Figure 1

Generation of sting^(−/−) zebrafish. (A) Schematic illustration of organization of zebrafish sting. The white and black boxes represent untranslated regions and open reading frames, respectively. The blue arrowhead indicates the single guide RNA (sgRNA) target site. The PAM and the sgRNA target sequences are indicated in red and blue letters, respectively. (B) The obtained mutation in sting^(−/−) fish using CRISPR/Cas9 gene editing. Deleted nucleotides in the sting^(−/−) genome are indicated with hyphens. (C) Amino acid prediction of Sting in the sting^{(−/− )} and WT zebrafish genomes. The altered amino acids are represented with red letters. (D) Confirmation of mutation via RT–PCR using sting^(−/−) mutation-specific primers in 7 dpf larvae of sting^(−/−) and WT zebrafish.