Fig. 5.

Glucosylsphingosine is bactericidal in vivo. (A) Bacterial burden measured by fluorescence in gba1^sa1621/+ incross larvae treated with 0.5 μM carmofur or vehicle control 5 dpi after CV infection with 200 to 300 Mm. Horizontal bars, means; ns, not significant; *P < 0.05; **P < 0.01 (one-way ANOVA with Tukey’s posttest). Representative of two independent experiments. (B) GlcSph (pmol/fish) in asah1b incross larvae that are either wild type or G0 crispant for gba1. Mean ± SD; ns, not significant; **P < 0.01; ****P < 0.0001 (one-way ANOVA with Tukey’s posttest). Representative of two independent experiments. (C) Bacterial burden measured by fluorescence in asah1b incross larvae that are either wild type or G0 crispant for gba1 5 dpi after CV infection with 200 to 300 Mm. Horizontal bars, means; ns, not significant; *P < 0.05 (one-way ANOVA with Tukey’s posttest). (D) Representative confocal images showing phagosome-localized Mm (green) and phagolysosome-localized Mm (green plus magenta) inside zebrafish macrophages (bright field, dashed lines) at 12 hpi. (Scale bar, 10 μm.) (E) Percentage of animals in which the single infecting Mm bacterium was localized to a phagosome or a phagolysosome; ns, not significant (Fisher’s exact test). Representative of two to three independent experiments. (F) Percentage of infected wild-type and gba1 G0 crispants at 5 dpi after HBV infection with a single Mm bacterium. P, phagosome; PL, phagolysosome; ns, not significant; *P < 0.05 (Fisher’s exact test). Representative of two to three independent experiments.