Fig. 3

a Top: Analytical RP-HPLC profiles of HwTxIV-Nvoc after different durations of illuminations (365 nm, 45 mW/cm²) demonstrating time-dependent control of uncaging. Bottom: Uncaged/Caged ratio of HPLC chromatogram peaks area versus irradiation time at 365 nm (45 mW/cm²). b Top: Analytical RP-HPLC profiles of HwTxIV-Nvoc after different intensities of illuminations (365 nm, 5 min) demonstrating intensity-dependent control of uncaging. Bottom: Uncaged/Caged ratio of HPLC chromatogram peaks area versus irradiation power at 365 nm (3 min). c Analytical RP-HPLC profiles of non-caged HwTxIV (top), 50:50 ratio of noncaged HwTxIV after partial uncaging of HwTxIV-Nvoc (middle) and of HwTxIV-Nvoc (bottom). d Mass analyses of noncaged HwTxIV and uncaged HwTxIV by LC-ESI QTOF ([M + 6H]⁶⁺ values). e Superimposition of TOCSY spectra (same area as Fig. 1e) of the noncaged HwTxIV (in black), and of the uncaged HwTxIV after illumination of HwTxIV-Nvoc (in purple). f Representative recording of hNa_V1.6 currents elicited at 10 mV from a holding potential of -100 mV illustrating the extent of current block by different concentrations of non-caged HwTxIV (black) and uncaged HwTxIV (purple). Scales: 2 ms, 1 nA. g Average dose-response curves for hNa_v1.6 current inhibitions by non-caged HwTxIV (black) and uncaged HwTxIV (purple). The data were fitted according to a Hill equation (noncaged HwTxIV IC₅₀ = 22.4 nM (n = 5) and uncaged HwTxIV IC₅₀ = 15.20 nM (n = 6)). Data are presented as mean ± SEM. Source data are provided as a Source Data File.