Fig. 4

Fig. 4. The protective effect of YIAEDAER on DA neurons and blood vessels in zebrafish PD model. (A) Representative fluorescence images of Vmat2: GFP zebrafish. Display enlarged image to improve the visualization of DA neuron morphology, red brackets indicate DA neurons. Scale bar, 100 µm. (B) The length of DA neurons in different treatment groups (ratio to control, %) (n = 8). *** P < 0.001 vs Ctl; ^##P < 0.01, ^###P < 0.001 vs MPTP. (C⁾ Fluorescence of DA neuron region (ratio to control, %) (n = 8). *** P < 0.001 vs Ctl; ^#P < 0.05, ^###P < 0.001 vs MPTP. (D) Representative fluorescence images of Fli1: GFP zebrafish in different treatment groups, red arrows indicate the loss of cerebral vessels. Co-treatment with YIAEDAER peptides protected the loss of vasculature and disorganized vasculature induced by MPTP as indicated by blue arrows. Scale bar, 100 µm. (E) Statistical analysis of the number of integral blood vessels in the brain (n = 8). *** P < 0.001 vs Ctl; ^#P < 0.05, ^##P < 0.01 vs MPTP.