Fig. 1
- ID
- ZDB-FIG-230224-4
- Publication
- Balamurugan et al., 2022 - PHLPP1 promotes neutral lipid accumulation through AMPK/ChREBP-dependent lipid uptake and fatty acid synthesis pathways
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ChREBP-mediated PHLPP1 regulation in in vitro foam cells (A–C) Dose and time kinetics of PHLPP1 protein levels (A) with densitometric quantification (bottom) and mRNA (B and C) levels after OxLDL treatment in RAW 264.7 cells; Statistical analysis was performed using one-way ANOVA followed by Bonferroni multiple comparisons test for (C) (∗p < 0.05 vs Control, 0 h; ap < 0.05 vs 24 h). (D) Protein levels of PHLPP1, ChREBP, and ER stress markers upon 7-ketocholesterol treatment under different time points. Densitometric analysis is shown in Figure S1C. (E) PHLPP1 protein levels upon 48 h OxLDL and 7-ketocholesterol treatment along with densitometric quantification (Bottom). (F and G) PHLPP1 protein (F) and mRNA (G) levels upon ChREBP overexpression; Statistical analysis was performed using two-tailed t-test for (G) (∗p < 0.05 vs Vector). (H) PHLPP1 and FASN protein levels in ChREBP knockdown cells treated with 7-kC or OxLDL. Densitometric analysis is shown in Figure S1D. (I) Recruitment of ChREBP to PHLPP1 promoter. Data are a representative of three independent experiments. Numerical data are expressed as mean ± SEM. See also Figures S1 and S2. |