Fig. 4

GCEP cells have invasion, migration, and proangiogenic capabilities. Transversal 15 mm sections at the trunk level of  fli-1 zebrafish larvae after 6 dpi with 50 GCEP cells. A, E H&E-stained sections; dashed line in 4A shows an aberrant vessel or a group of vessels, and black arrowhead shows cells moving from the yolk to the intestinal bulb (IB), inset in E. B–D Confocal images of transversal sections showing CM-Dil-stained GCEP cells in orange, cyan marking endothelial cells from a Tg(fli-1:EGFP)^y1 zebrafish larvae, and Hoechst stained nuclei in light gray. Pink arrowheads indicate the large vessel observed in panel A, B. White arrows and arrowheads show injected cells with high and low fluorescence intensity, respectively. C, D insets indicated in panel B, dashed line in panel D showing an irrigated tumor. F side view of a larvae indicating sectioning site. All scale bars indicate 100 μM. Images were acquired with a Zeiss confocal microscope LSM800 or Axio Zoom V16, stereomicroscope