Fig. 7

(Note: the relative velocity and acceleration periodicity data mimicked the patterns observed in relative movement; see Supplementary Fig. 4). Gray = control (n = 248 fish), blue = CuSO₄ (n = 204 fish), green = neomycin (n = 222 fish). Spectral decomposition of the observed data (a, d, g) removed the noise (Supplementary Fig. 3g, j, k) to reveal the overall underlying trends (b, e, h) and the periodicity, or recurring fluctuations (c, f, i) that occurred during any given 1 s of the experiment. The periodicity waveform peaks (c, f, i) indicate the average amount (amplitude), number, direction (positive = increasing; negative = decreasing), and order of occurrence for these cyclic fluctuations as a function of unit time (1 s). The overall trends were that CuSO₄ treated fish had the least relative movement (b), while the control fish more rapidly oriented to 0° (e) and swam with more angular variance (h; 1 – mean length of the resultant vector) compared to lesioned fish. The periodic fluctuation in relative distance moved (c) was greatest in CuSO₄ treated fish compared to control or neomycin treated fish. However, the fluctuation in mean body angle (f) was greatest in neomycin treated fish compared to control and CuSO₄ fish, while the fluctuation in mean length of the resultant vector (i.e the angular variance; i) was greatest in control fish compared to lesioned fish.