FIGURE

Fig. 2

ID

ZDB-FIG-230120-3

Publication

Adane et al., 2021 - STAG2 loss rewires oncogenic and developmental programs to promote metastasis in Ewing sarcoma

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Fig. 2

STAG2 occupies PRC2- and enhancer-marked regions, and its deletion is incompletely compensated by STAG1

(A) Genome-wide heatmaps of SA2, SA1, and SMC1A ChIP-seq peak centered signal in A673 cells expressing WT or KO STAG2. Regions are ranked based on WT signal.

(B) Read density metaplots showing average RPKM (reads per kilobase per million)-normalized signal for SA2, SA1, and SMC1A in WT (black) and STAG2 KO (red) A673 cells. Differential read density in KO versus WT conditions. Unpaired t test with Welch's correction, ^∗∗∗∗p < 0.0001; ns, not significant.

(C) Clustered heatmaps of ChIP-seq peak centered signal for SA2, SA1, SMC1A, H3K27ac, H3K27me3, chromatin accessibility (ATAC-seq), and H3K4me3. Cluster regions are ranked by SA2 signal.

(D) Hockey plots depicting motifs enriched in SA2/H3K27ac enhancers and SA2/H3K27me3 regions defined in (C).

(E) Clustered heatmaps depicting SA2, SA1, and SMC1A signal in the cohesin regions defined in (C) in control and STAG2 KO A673 cells.

(F) Metaplots showing average SA1 signal in the cohesin regions defined in (C). Differential read density in KO versus WT conditions. Unpaired t test with Welch's correction, ^∗∗∗∗p < 0.0001, ^∗∗p < 0.01.

(G) Clustered heatmap depicting EWS/FLI1 signal in cohesin regions defined in (C) in control and STAG2 KO A673 cells.

(H) Metaplot showing average EWS/FLI1 signal in cohesin regions defined in (C). Differential read density in KO versus WT. Unpaired t test with Welch's correction, ^∗∗∗∗p < 0.0001, ^∗∗p < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cancer Cell, 39, Adane, B., Alexe, G., Seong, B.K.A., Lu, D., Hwang, E.E., Hnisz, D., Lareau, C.A., Ross, L., Lin, S., Dela Cruz, F.S., Richardson, M., Weintraub, A.S., Wang, S., Iniguez, A.B., Dharia, N.V., Conway, A.S., Robichaud, A.L., Tanenbaum, B., Krill-Burger, J.M., Vazquez, F., Schenone, M., Berman, J.N., Kung, A.L., Carr, S.A., Aryee, M.J., Young, R.A., Crompton, B.D., Stegmaier, K., STAG2 loss rewires oncogenic and developmental programs to promote metastasis in Ewing sarcoma, 827-844.e10, Copyright (2021) with permission from Elsevier. Full text @ Cancer Cell