FIGURE

Fig. 4.

ID

ZDB-FIG-230119-24

Publication

Laureano et al., 2022 - shox2 is required for vestibular statoacoustic neuron development

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Fig. 4.

Comparing number of SAG between shox2^+/+ and shox2^Δ/Δ animals. HuC/D immunostaining of shox2^+/+ and shox2^Δ/Δ larvae that contain the Tg(neurod1: EGFP) reporter at (A) 2, (B) 3 and (C) 5 dpf. EGFP (green) and HuC/D (magenta) are shown. Asterisks mark the anterior SAG (aSAG). (D) HuC/D cell counts from aSAG are as follows: 2 dpf, shox2^+/+ (55.7±3.0 cells, n=12 larvae), shox2^Δ/Δ (51.2±2.4 cells, n=11 larvae), 3 dpf, shox2^+/+ (66.4±3.1 cells, n=10 larvae), shox2^Δ/Δ (56.1±3.1 cells, n=11 larvae), 5 dpf, shox2^+/+ (63.2±1.8 cells, n=11 larvae), shox2^Δ/Δ (55.1±2.1 cells, n=12 larvae). (E) HuC/D cell counts from posteromedial SAG (pSAG) are as follows: 2 dpf, shox2^+/+ (39.5±1.9 cells, n=15 larvae), shox2^Δ/Δ (39.1±1.8 cells, n=14 larvae), 3 dpf, shox2^+/+ (38.9±3.8 cells, n=10 larvae), shox2^Δ/Δ (43.9±1.9 cells, n=11 larvae), 5 dpf, shox2^+/+ (58.6±3.7 cells, n=13 larvae), shox2^Δ/Δ (55.4±4.3 cells, n=14 larvae). One-way ANOVA and Sidak multiple comparison test were used. *P≤0.05, **P≤0.01. Values reported as mean±s.e.m. Scale bars: 10 µm.

Expression Data

Gene:	EGFP
Antibody:	Ab1-elavl
Fish:	nl1Tg shox2^nj3/nj3; nl1Tg
Anatomical Terms:	statoacoustic (VIII) ganglion statoacoustic (VIII) ganglion neuron
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	shox2^nj3/nj3; nl1Tg
Observed In:	neuron statoacoustic (VIII) ganglion anterior region statoacoustic (VIII) ganglion posterior region
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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