FIGURE
Fig. 4
- ID
- ZDB-FIG-230118-11
- Publication
- Yates et al., 2021 - A simple and rapid method for enzymatic synthesis of CRISPR-Cas9 sgRNA libraries
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Fig. 4
GFP Knockout Screen. (A) Genomic DNA was isolated from HeLa cells stably expressing GFP-LC3. The four HpaII sites within the GFP ORF and the predicted sgRNAs are labeled. (B) FACS sorting of transfected and untransfected cells. GFP fluorescence is on the x-axis and counts are on the y-axis. (C) Mutation frequency at each of the HpaII sites based on high-throughput sequencing of the GFP locus. (D) The most common variants for each of the four HpaII sites. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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