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Fig. 4
GFP Knockout Screen. (A) Genomic DNA was isolated from HeLa cells stably expressing GFP-LC3. The four HpaII sites within the GFP ORF and the predicted sgRNAs are labeled. (B) FACS sorting of transfected and untransfected cells. GFP fluorescence is on the x-axis and counts are on the y-axis. (C) Mutation frequency at each of the HpaII sites based on high-throughput sequencing of the GFP locus. (D) The most common variants for each of the four HpaII sites.
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