FIGURE

Figure 1

ID

ZDB-FIG-230110-47

Publication

Hayashiji et al., 2022 - α-1,6-Fucosyltransferase Is Essential for Myogenesis in Zebrafish

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Figure 1

Zebrafish injected with fut8a MO showed abnormalities in skeletal muscle (a) Lectin blot, with AAL, of Zebrafish morphants injected with 3 ng of CMO or fut8a MO at 2 dpf. Total protein was visualized by Coomassie Blue staining and Ponceau staining (Supplemental Figure S1a,b): Histogram of the percentage of normal, abnormal, and dead fish in non-injected fish, CMO and fut8a MO injected fish at 2 dpf. Blue: normal, orange: abnormal, gray: dead. (c,d) Comparison of the CMO and fut8a MO injected zebrafish under bright field (c) and birefringence assay (d) at 2 dpf. d: Upper bar = 1 mm; lower bar = 200 ?m. Non: non-injected zebrafish.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-fut8a
Knockdown Reagent:	MO1-fut8a
Observed In:	skeletal muscle spinal cord whole organism whole organism N-glycan fucosylation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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