FIGURE

Figure 3

ID

ZDB-FIG-230110-11

Publication

Chen et al., 2022 - A Novel Antimicrobial Peptide Sp-LECin with Broad-Spectrum Antimicrobial Activity and Anti-Pseudomonas aeruginosa Infection in Zebrafish

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Figure 3

Effect of Sp-LECin on the membrane permeability of P. aeruginosa and A. baumannii. (A,B) Outer membrane permeability after Sp-LECin treatment was measured by N-phenyl-1-naphthylamine (NPN) uptake assay. The NPN fluorescence was recorded at the excitation and emission wavelengths of 350 and 420 nm, respectively. (C,D) Inner membrane permeability after Sp-LECin treatment was measured by SYTOX Green uptake assay. The SYTOX Green fluorescence was recorded at the excitation and emission wavelengths of 485 and 530 nm, respectively. The bars indicate the mean � standard error of mean (n = 3). (E,F) Confocal laser-scanning microscope (CLSM) images representing the cell membrane permeability by SYTO 9 and propidium iodide (PI) staining in P. aeruginosa and A. baumannii cells treated with Sp-LECin.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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