FIGURE

Figure 4

ID

ZDB-FIG-221230-13

Publication

Stenzel et al., 2022 - Distinct and redundant roles for zebrafish her genes during mineralization and craniofacial patterning

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Figure 4

Osteoblasts differentiate in a spatiotemporal pattern similar to wild types in her9 mutants, but only weakly mineralize bone. sp7:EGFP;her9 heterozygotes were crossed to her9 heterozygotes and offspring were sorted for transgene expression. 16 live transgenic animals were labeled with Alizarin Red and imaged at 3 dpf. After imaging, animals were recovered and grown in individual wells until 6 dpf when they were labeled with Alizarin Red and imaged again. Individual animals were recovered for genotyping to identify homozygous wild types (A) and homozygous her9 mutants (B) that were imaged twice. The following structures are indicated: opercle bone (op), branchiostegal ray (br), cleithrum (cl), otoliths (ot). Arrows indicate osteoblasts prominently detected along the ventral edge of the opercle at 6 dpf. Scale bar is 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	her9^co66/co66; b1212Tg/b1212Tg
Observed In:	opercle opercle bone mineralization osteoblast otolith otolith mineralization
Stage Range:	Protruding-mouth to Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)