FIGURE

FIGURE 2

ID

ZDB-FIG-221227-2

Publication

Sun et al., 2022 - Craniofacial and cardiac defects in chd7 zebrafish mutants mimic CHARGE syndrome

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FIGURE 2

Overall phenotypes of chd7 mutants. (A) Expression of chd7 in chd7 ^sr5 and chd7 ^sr6 larvae at 4 dpf. The fold change of chd7 expression compared to WT was shown. (B) Percentage of the surviving offspring from chd7 ^sr5incross (n > 40 for each time point). (C) Bright field images of chd7 ^sr5 and MZchd7 ^sr5 mutants at 7 dpf. MZchd7 ^sr5 fish have a smaller swim bladder (yellow arrowhead), pericardial edema (red asterisk), and small eyes (blue arrowhead). (D) Quantification of heart edema. Nearly 40% of MZchd7 ^sr5 fish have heart edema; however, chd7 ^sr5 fish do not show heart edema phenotypes. WT: n = 879, chd7 ^sr5: n = 747, MZchd7 ^sr5: n = 751. (E) Quantification of the small eye phenotype. The longest diameter of the dissected eye was measured. The eye size of the MZchd7 ^sr5 mutants is significantly reduced (n = 22 for each group). Statistical analyses were performed using the ordinary one-way ANOVA test for (A,E) and chi-square test for (B,D). ns: p ≥ 0.05, *: p < 0.05, **: p < 0.01, ***: p < 0.001, and ****: p < 0.0001.

Expression Data

Gene:	chd7
Fish:	AB chd7^sr5/sr5 chd7^sr6/sr6 chd7^sr5/+ chd7^sr6/+
Anatomical Term:	whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	chd7^sr5/sr5 chd7^sr5/sr5 chd7^sr6/sr6 chd7^sr5/+ chd7^sr6/+
Observed In:	eye pericardium swim bladder whole organism
Stage Range:	Day 4 to Days 7-13

Phenotype Detail

Acknowledgments

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