Fig. 1

a Robotic tissue micromanipulation platform along with the stereo microscope and operation chamber. b Schematic illustration of the adapters designed to hold actuated and non-actuated instruments (not to scale). c Schematic showing the zebrafish embryo from different anatomical axes (V/D: ventral/dorsal, A/P: anterior/posterior, L/R: left/right). d A representative bright field (BF) image of a zebrafish embryo. Tissues that are studied in this work are indicated on the embryo. e Line of interest indicated with blue is generated to measure the AP tail length from BF image shown in (d). f Composite images of the embryo showing BF and Her1-YFP channels at different time points. g A BF image of the embryo right after robot-assisted microsurgery. h Light-sheet fluorescence image of a tail explant from a utr-mCherry transgenic line which marks filamentous actin structures. White dashed lines indicate the plane at which ventral and dorsal-view images were taken. White arrows indicate the somites, blue dashed-lines indicate notochord (Noto: notochord). i Composite images of a tail explant over time showing the elongation of the tail along with Her1-YFP signal. Scale bars, 100 μm.