FIGURE

Fig. 6

ID

ZDB-FIG-221223-6

Publication

Lei et al., 2021 - Noncanonical protease-activated receptor 1 regulates lymphatic differentiation in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Overexpression of cleaved par1 recovers the lymphatic phenotypes observed in mmp13b zebrafish mutants

(A) Confocal images showing TD formation of siblings and mmp13b homozygous mutants in the Tg(lyve1b:mcherry) line and Tg(lyve1b:cleaved-par1-P2A-mcherry) line at 5 dpf. Blue arrowheads indicate TD formation in each somite; yellow asterisks represent the absence of TD formation in each somite. Scale bars: 100 μm.

(B) Percentage of somites lacking TD formation in siblings with the Tg(lyve1b:mcherry) line (n = 30 embryos), mmp13b homozygous mutants with the Tg(lyve1b:mcherry) line (n = 20 embryos), siblings with the Tg(lyve1b:cleaved-par1-P2A-mcherry) line (n = 30 embryos), and mmp13b homozygous mutants with the Tg(lyve1b:cleaved-par1-P2A-mcherry) line (n = 20 embryos); 6 somites/embryos were used for quantification. In (B), values represent means ± SEMs. ∗p ≤ 0.01, ∗∗p ≤ 0.001 in the Student's t test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience