ZFIN Image: Lei et al., 2021, Fig. 6

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Figure Caption

Fig. 6

Overexpression of cleaved par1 recovers the lymphatic phenotypes observed in mmp13b zebrafish mutants

(A) Confocal images showing TD formation of siblings and mmp13b homozygous mutants in the Tg(lyve1b:mcherry) line and Tg(lyve1b:cleaved-par1-P2A-mcherry) line at 5 dpf. Blue arrowheads indicate TD formation in each somite; yellow asterisks represent the absence of TD formation in each somite. Scale bars: 100 μm.

(B) Percentage of somites lacking TD formation in siblings with the Tg(lyve1b:mcherry) line (n = 30 embryos), mmp13b homozygous mutants with the Tg(lyve1b:mcherry) line (n = 20 embryos), siblings with the Tg(lyve1b:cleaved-par1-P2A-mcherry) line (n = 30 embryos), and mmp13b homozygous mutants with the Tg(lyve1b:cleaved-par1-P2A-mcherry) line (n = 20 embryos); 6 somites/embryos were used for quantification. In (B), values represent means ± SEMs. ∗p ≤ 0.01, ∗∗p ≤ 0.001 in the Student's t test.

Acknowledgments

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