Fig. 3

Temporal concurrence of mitosis and MOWs. (A-C) Visualization of blastomere/eYSN cycle phases (relative ROI indicated in panel (G)) through microtubules’ labelling: (A) metaphase, (B) interphase, (C) metaphase (at minutes 9, 24, 102). Scale bars 25 µm. (D-F) Visualization of MOWs passages in the subsequent yolk region (relative ROI indicated in panel (G)). The MT network is (D) highly oriented and sparse, then (E) re-diffuses, and again (F) sparse (at minutes 15, 27, 108). Scale bars 25 µm. (G-H) The same dclk2-GFP embryo from a lateral view at three different timepoints (at minutes 9, 24, 102), scale bars 200 µm. Animal pole at top left, vegetal pole at bottom right. Arrows indicate expansion (pointing outward in A and C) and contraction (pointing inward in B) of the blastoderm margin. (J) Normalized mean intensity graph visualizing blastomeres/YSNs cycles (metaphases are peaks, interphases are valleys, graph obtained from the local analysis). (K) Normalized mean intensity graph visualizing the periodic MOWs passage (arrows) over the yolk region (graph obtained from the local analysis). (L) The periodic blastoderm contractions (valleys) and expansions (peaks). Blastoderm margin expansion and nuclei metaphase appear to be synchronized, MOW passage follows after few minutes. See Supplement 1 for details on how the graphs were retrieved. See also Visualization 2.