FIGURE

FIGURE 2

ID

ZDB-FIG-221211-76

Publication

Zhang et al., 2022 - In situ assessment of statins' effect on autophagic activity in zebrafish larvae cardiomyocytes

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FIGURE 2

The density of autophagosomes in zebrafish heart is regulated by known autophagy-modulating drugs. (A) Representative images of 3-dpf zebrafish heart of Tg(myl7:EGFP-LC3) line when treating with indicated drugs for 24 h. The top row shows the Z-stack maximum projections (scale bar: 50 μm), the bottom row shows the single slice (scale bar: 10 μm), inset in the bottom row shows the single punctum in indicated treated groups (scale bar: 3 μm). Arrowhead represents the autophagosome, double arrow line represents the diameter of the autophagosome. (B) Quantification of the density of autophagosomes in 3-dpf zebrafish heart of Tg(myl7:EGFP-LC3) line when treating with indicated drugs for 24 h. The data are shown as mean ± s.e.m for each group. n = 18 in control group, n = 55 in 3MA group, n = 58 in BafA group, n = 65 in P/E group, and n = 36 in Rapamycin group. (C) Quantification of the diameter of puncta in 3-dpf zebrafish heart when treated with BafA compared with the control group. n = 50 in the control group, and n = 50 in the BafA group. ***p < 0.001. A, atrium; V, ventricle.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cardiovasc Med