Figure 5

Combined exposure of Cb2r with CBN prevents the CBN-induced deficits in locomotion. The free swimming movement of the fish was recorded for 60 min and the recorded video was analyzed later. Tracing was recorded at 5 dpf for vehicle control (n = 18), 3 mg L⁻¹ CBN (n = 25), AM251 10 nm + 3 mg L⁻¹ CBN (n = 37), CP94 10 nm + 3 mg L⁻¹ CBN (n = 31), AM630 10 µm + 3 mg L⁻¹ CBN (n = 32), JTE907 10 µm + 3 mg L⁻¹ CBN (n = 37), AM251 10 nm + AM630 10 µm + 3 mg L⁻¹ CBN (n = 36) and Cp94 10 nm + JTE907 10 µm + 3 mg L⁻¹ CBN (n = 40) respectively. N = 4 experiments. (A) Bar graph shows the mean frequency of swim bouts within 1 h. (B) Bar graph shows the mean swimming activity (%) in 1 h. (C) Bar graph shows the mean swimming velocity (mm s⁻¹) over a 1 h time period. Groups which share the same letter(s) of the alphabet are not statistically different from one another; p < 0.05.