Figure 4

Combined exposure of Cb2r and CBN inhibits CBN-induced changes in primary motor neuron branching. (A) Znp-1 (green) antibody was used to label primary motor neuron axons and their branches in vehicle control (n = 7), 3 mg L⁻¹ CBN (n = 9), AM251 10 nm + 3 mg L⁻¹ CBN (n = 8), CP94 10 nm + 3 mg L⁻¹ CBN (n = 9), AM630 10 µm + 3 mg L⁻¹ CBN (n = 9), JTE907 10 µm + 3 mg L⁻¹ CBN (n = 8), AM251 10 nm + AM630 10 µm + 3 mg L⁻¹ CBN (n = 5) and Cp94 10 nm + JTE907 10 µm + 3 mg L⁻¹ CBN (n = 6). Dorsal and ventral branches in a motor axon are indicated with (D) and (V) respectively, and the white arrow points to secondary branches in the vehicle image. N = 3 experiments; Scale bar represents 25 µm. (B,C) Bar graph shows the number of secondary branches in the dorsal and ventral regions of the trunk. N = 3 experiments. Groups which share the same letter(s) of the alphabet are not statistically different from one another; p < 0.05.