FIGURE 7
- ID
- ZDB-FIG-221118-170
- Publication
- Ikkala et al., 2022 - Zebrafish cornea formation and homeostasis reveal a slow maturation process, similarly to terrestrial vertebrates' corneas
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EdU/BrdU double-labeling on zebrafish cornea. (A) EdU (green) was administered to the fish for 24 h, followed by a chase period of 3?15 days. Then, BrdU (red) was administered for 24 h, and samples were collected. (B) EdU (green) and BrdU (red) staining on 5 µm paraffin sections. The panel shows the overview of the anterior eye, and central as well as peripheral/limbal regions of the cornea. (C) Quantification of EdU+, BrdU+, double positive, and EdU/BrdU-negative cells on the central region (left), and the peripheral region (right). Results represent mean ± SD (n = 6?9). The mean value of 2?3 sections from the middle of the eye were used per fish. Scale bars: 20 µm. CC, central regions; PC, peripheral/limbal region. |