FIGURE

Figure 3

ID

ZDB-FIG-221118-158

Publication

Mignani et al., 2022 - Bi-Allelic Mutations in Zebrafish pank2 Gene Lead to Testicular Atrophy and Perturbed Behavior without Signs of Neurodegeneration

Other Figures

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Figure 3

Oxygen consumption rate (OCR) and cell death analysis. (A) Oxygen consumption rate (OCR) in control (Ctl) and mutant (D4) embryos at 48 hpf (Ctl N = 9; D4 N = 10) and (B) at 72 hpf (Ctl N = 9; D4 N = 10). 1 = basal rate; 2 = 10 μM oligomycin, 3 = 1 μM FCCP; 4 = 2 μM FCCP; 5 = 1 μM rotenone and antimycin A. * p < 0.05, Student’s t-test. (C) Lateral view of acridine orange stained embryos at 48 hpf. The numbers at the bottom of the images indicate the ratio between embryos with the observed phenotype and the total number of stained embryos. Size bar = 100 µm. (D) Quantification of acridine orange positive cells in the brain region (Ctl N = 33, D4 N = 32; two biological replicates).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pank2^ubx1/ubx1
Observed In:	aerobic respiration head apoptotic process
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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