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Fig. 1

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ZDB-FIG-221109-53

Publication

Svara et al., 2022 - Automated synapse-level reconstruction of neural circuits in the larval zebrafish brain

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Fig. 1

Pretectal 2P calcium imaging and whole-brain larval SBEM dataset acquisition.

a, Illustrative larval zebrafish head at 5 dpf, brain highlighted in red. b, Wire-frame representation of a 5 dpf zebrafish brain, slice stack represents location of the 2P calcium imaged volume centered on the pretectum. c,d, X-ray image (c) of sample used to calculate horizontally stacked tile pattern (d), shown here overlaid on a low-resolution vEM overview image. e, High-resolution vEM imaged brain slice stitched from individual tiles shown in d. f, The resulting high-resolution volume. g, Synaptic contact in pretectum, as highlighted in e. Scale bars, 50 μm (d–f), 500 nm (g).

Expression Data

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Antibody Labeling

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Methods