ZFIN Image: Svara et al., 2022, Fig. 1

Image

Figure Caption

Fig. 1 Pretectal 2P calcium imaging and whole-brain larval SBEM dataset acquisition.

a, Illustrative larval zebrafish head at 5 dpf, brain highlighted in red. b, Wire-frame representation of a 5 dpf zebrafish brain, slice stack represents location of the 2P calcium imaged volume centered on the pretectum. c,d, X-ray image (c) of sample used to calculate horizontally stacked tile pattern (d), shown here overlaid on a low-resolution vEM overview image. e, High-resolution vEM imaged brain slice stitched from individual tiles shown in d. f, The resulting high-resolution volume. g, Synaptic contact in pretectum, as highlighted in e. Scale bars, 50 μm (d–f), 500 nm (g).

Acknowledgments

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