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FIGURE 1

ID

ZDB-FIG-221109-1

Publication

Pardon et al., 2022 - Modulation of sleep behavior in zebrafish larvae by pharmacological targeting of the orexin receptor

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FIGURE 1

Behavioral analysis of zebrafish larvae. Zebrafish larvae were placed separately in a 96-well plate and incubated with compound or vehicle (VHC, 1% DMSO in embryo medium). After incubation, the 96-well plate was placed in an automated tracking device and larval behavior was recorded for varying times by the Zebralab software. The night assay consisted of a 2 h incubation and habituation period, followed by a 12 h recording period with a 1 h light phase from 8 p.m to 9 p.m., a 10 h dark phase from 9 p.m to 7 a.m., and again a 1 h light phase from 7 a.m to 8 a.m. The dark-light assay consisted of a 2 h 30 min incubation and habituation period, followed by a 2 h recording period with alternating 30 min dark-light triggers during the day. Figure created with BioRender.com.

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Antibody Labeling

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Pharmacol