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FIGURE 1
Behavioral analysis of zebrafish larvae. Zebrafish larvae were placed separately in a 96-well plate and incubated with compound or vehicle (VHC, 1% DMSO in embryo medium). After incubation, the 96-well plate was placed in an automated tracking device and larval behavior was recorded for varying times by the Zebralab software. The night assay consisted of a 2 h incubation and habituation period, followed by a 12 h recording period with a 1 h light phase from 8 p.m to 9 p.m., a 10 h dark phase from 9 p.m to 7 a.m., and again a 1 h light phase from 7 a.m to 8 a.m. The dark-light assay consisted of a 2 h 30 min incubation and habituation period, followed by a 2 h recording period with alternating 30 min dark-light triggers during the day. Figure created with