FIGURE

Fig 7

ID

ZDB-FIG-221105-8

Publication

Li et al., 2022 - Mycn regulates intestinal development through ribosomal biogenesis in a zebrafish model of Feingold syndrome 1

Other Figures

All Figure Page

Back to All Figure Page

Fig 7

Blockage of mTOR pathway and impaired protein translation results to intestinal defects in mycn mutants.

(A) Schematic representation of the experimental workflow of the Ribo-seq. (B) KEGG analysis of the genes showing reduced translation efficiency in the mycn mutant. The red box marks the mTOR pathway. (C) Detection of the rps6, phosphorylated rps6, eIF4EBP, and phosphorylated eIF4EBP in WT and mycn mutant embryos at 3 dpf. The proteins were detected by western blot. The tubulin expression level was used as internal control. (D) Inhibiting the mTOR pathway by different doses of rapamycin mimicked the intestinal defects phenotype of the mycn mutants. (E, F) Activating the mTOR pathway by injecting rheb mRNA and L-Leu treatment partially rescued the intestinal defects phenotype of the mycn mutants. All embryos are shown in dorsal view. Scale bar: 50 μm (C), 200 μm (D-F). Raw images of this figure are provided in S1 Raw Images. dpf, days postfertilization; ifabp, intestine fatty acid–binding protein; WT, wild-type.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.