Figure 6

Anti-inflammatory effect of PEs against CuSO₄-induced inflammation in vivo. (a) Effective concentrations of PEs and anti-inflammatory efficacies. (b) The fluorescence images of zebrafish embryos after treatment with 1 µM CuSO₄ alone or together with PEs at different concentrations. (c) The number of neutrophils in transgenic zebrafish embryos under different treatment conditions, ^∗∗∗p < 0.001 compared with the nontreatment group, and ^#p < 0.05, ^###p < 0.001 compared with the group treated with CuSO₄ alone (a-c). The expression of inflammation-related genes (IL-6, COX-2) and eicosanoid pathway-associated genes (COX-2, C3a, and PLA2) in vivo. The zebrafish embryos were exposed to CuSO₄ either with PEs or not for 2h. After, embryos were collected for qRT-PCR analysis. A pool of 30 embryos per group was used. Experiments were performed in triplicates. ^∗p < 0.05, ^∗∗∗p < 0.001 compared to the experiment treated with CuSO₄ alone (d-g).