FIGURE

Fig. 3

ID

ZDB-FIG-221101-14

Publication

Ulloa et al., 2021 - Definitive hematopoietic stem cells minimally contribute to embryonic hematopoiesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Thymic T cell contribution by embryonic progenitors and HSCs is distinct

(A) Experimental schema of 4-OHT-inducible lineage tracing to examine larval T cell production in the thymus from Prog+− and HSC-labeled cohorts.

(B and C) Fluorescent images of 4-OHT-induced switch (left) and EtOH non-switched controls (right) Tg(drl:creER^T2;ubi:Switch) larvae for Prog+ (B) and HSC (C) populations. Top images are 5 dpf larvae, and bottom images are 10 dpf larvae. Dashed box and inset showing the thymus where T cells colonize. mCherry⁺ fluorescence corresponds to drl+ switched daughter cells. Scale bars, 500 μm. Representative images are shown, with quantification in (D).

(D) Quantification of mCherry fluorescence intensity in the thymic region in larvae of Prog+− and HSC-labeled cohorts measured over a time course of 5–16 dpf. Mean ± SEM of the mCherry⁺ corrected total cell fluorescence (CTCF) at each time point is shown. Two-way ANOVA with Sidak’s multiple comparison (n = 6–30 per larvae/day). *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. See also Figure S3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.