Figure 1

Clustering and pathway analysis of differentially expressed genes (DEGs). (A) Heatmap of significant DEGs between wild type and pax3a^−/−;pax3b^−/− zebrafish embryos. Regularized log transformed (rlog) count matrix was generated using DESeq2 package. Significant DEGs were used to plot the heatmap of rlog counts. Red and blue represent up- and down-regulated genes, respectively. (B) Scatter plot for DEGs in wild type and pax3a^−/−;pax3b^−/− showing mean of normalized counts and log2 fold change. All differentially expressed genes (in blue) with adjusted p-value < 0.01. (C) Disease ontology analysis of down-regulated genes. (D,E) KEGG pathway analysis of up- and down-regulated genes. Bar plots represents the number of DEGs involved in each condition. Adjusted p-value was represented by colour scale, and the statistically significance level decreased from red (highly significant) to blue (relatively lower significant). p-value < 0.01.