Figure 4

zAvatars were generated from naive rectal cancer biopsies. One day after injection zAvatars were submitted to 25Gy radiation dose (B, B’), 25Gy+5FU (C, C’) or 25Gy+MET (D, D’) regimens, and compared with control (A, A’) At 3dpi, apoptosis (F, I, K), tumor size (E, H, L) and metastatic potential (G, J, M) were analyzed. Tumor cells are labeled in white (Deep Red), activated Caspase3 in green (A’–D’), DAPI in blue and human-mitochondria marker in red. Images correspond to zAvatar#4, and quantifications are shown for zAvatar#4, #5 and #6. Data is expressed as mean ± SEM and each dot represents a xenograft. Dashed white line is delimitating the tumor of each xenograft. Scale bars represent 50µm. Statistical results: (ns) > 0.05, *P≦0.05, **P≦0.01. Results were compared with control with exception of those that have a bar to indicate the compared groups.