FIGURE

Fig. 7

ID

ZDB-FIG-220922-52

Publication

Mondal et al., 2022 - A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Figure 7. TGF-?2-mediated signaling regulates srGAP1^low phenotypes

(A) Matrix degradation of Ctrl and srGAP1 si #1 MDA-MB-231 cells with or without depletion of TGF-?2 by siRNA. Cells plated on fluorescent gelatin for 16 h. n = 51 (Ctrl), 47 (Ctrl + TGF-?2 siRNA), 52 (srGAP1 si #1), and 58 (srGAP1 si #1 + TGF-?2 siRNA) fields quantified. Data are from three independent experiments and represented as a violin plot, horizontal lines are median and interquartile range. Kruskal-Wallis test, corrected with Dunn?s test.

(B) Immunoblot of Ctrl and srGAP1 si #1 MDA-MB-231 cells with or without depletion of TGF-?2 by siRNA. Top blot: anti-srGAP1. Middle blot: anti-TGF-?2, ^? represents the TGF-?2 band. Bottom blot: loading control: anti-?-tubulin.

(C) Matrix degradation of Ctrl and srGAP1 si #1 MDA-MB-231 cells with DMSO or 5 ?M LY364947. Cells treated in serum-free medium with DMSO or 5 ?M LY364947 for 24 h, then plated on fluorescent gelatin for 16 h with DMSO or 5 ?M LY364947. n = 45 (Ctrl + DMSO), 38 (Ctrl +5 ?M LY364947), 52 (srGAP1 si #1 + DMSO), and 61 (srGAP1 si #1 + 5 ?M LY364947) fields quantified. Data are from three independent experiments and represented as a violin plot, horizontal lines are median and interquartile range. Kruskal-Wallis test, corrected with Dunn?s test.

(D) Representative images of cells seeding the lung after tail-vein injections. Left panel: GFP-labeled MDA-MB-231 tumor cells. Middle panel: fibrillar collagen (second harmonic generation). Right panel: merge, tumor cells in green and fibrillar collagen in red. Scale bar, 20 ?m.

(E) Area of tumor cells that have extravasated into the lung per field containing cells. GFP-labeled MDA-MB-231 Ctrl and srGAP1 si #1 MDA-MB-231 cells with or without depletion of TGF-?2 by siRNA. Cells injected into the tail-vein of mice. n = 31 (Ctrl), 31 (Ctrl + TGF-?2 siRNA), 29 (srGAP1 si #1), and 25 (srGAP1 si #1 + TGF-?2 siRNA) fields quantified, pooled over 5, 5, 4, and 5 mice, respectively. Data are represented as a box and whisker plot; horizontal lines are median and interquartile range. Kruskal-Wallis test, corrected with Dunnett T3 test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.