Fig. 5
Cortisol does not affect brain glucose uptake. (A) Uptake of the fluorescent glucose analogue 2-NBDG in the brain of the cortisol-treated fish remained unchanged compared to the control. (B) The transcript levels of the glucose transporters (slc2a’s) in the brain did not change after cortisol exposure. (C) Cortisol treatment did not change the ratio of phosphorylated to total AMPK (M.W.:62 kDa) in the brain. Values are means ± SEM (Relative fluorescence units; RFU n = 11–12; Transcript abundance n = 5–6; AMPK expression n = 6). ns—not significant; Bars with different letters are significantly different (one-way ANOVA; P ≤ 0.05). |