Fig. 2
Glucose reduces AMPK phosphorylation in the brain. (A) Glucose exposure decreased the ratio of phosphorylated to total AMPK (M.W.:62 kDa) in the brain compared to the control. (B) The transcript levels of the glucose transporters (slc2a’s) in the brain did not change after glucose exposure. As we were comparing relative abundance of all slc2a’s in the brain, we chose to normalize all slc2a’s to the slc2a2, which showed the lowest transcript abundance. (C) mRNA levels of glucokinase (gck) remained unchanged after glucose exposure when compared to the control. Values are means ± SEM. (AMPK expression n = 5–6; Transcript abundance n = 6). ns—not significant; *(P < 0.05). |