Fig. 6

Brain morphology during pupation stained with DAPI stain (blue); anti-phospho-histone H3 (anti-pH3, red). (a–d) Brains from wild type siblings’ pupae; (e,f) Df(1)14.4; Mi{ET1}CG7716^MB07394 Dp(1;3)DC364 PBac{y⁺w⁺} mutants. (a) Normal pupal brains 8 h after pupal case formation resemble larval brains, although the hemispheres elongate distally and show clear mitotic zones at the tips, marked by anti-pH3. (b) Further elongation is seen at 12 h, when normal brain and ventral nerve cord lose tight connection. (c) The majority of normal brains are dissected separately at this time point. (d) WT brains one day later at 36 h look different; the connection to eyes becomes visible; mitoses are not seen. Early stage mutant brains resemble wild type. (e) Mutant brains at 15 h (after early adult body parts are formed in normal pupae) and (f) at 36 h resemble “early 12 h wild type”. At 36 h, brain mitoses are barely detected. White bar = 100 μm.