FIGURE

Fig 6

ID

ZDB-FIG-220909-51

Publication

Kaufman et al., 2018 - CORRECTION: rbpms2 functions in Balbiani body architecture and ovary fate

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Fig 6

RNA-binding domain is required for Rbpms2 localization in germ cells.

(A) Schematic representing constructs used in granule localization assays. (B) Schematic representing transgenic constructs used to express Rbpms2b in oocytes. (C-C”) Wild-type GFP-Rbpms2b localizes with Vasa in germ granules of PGCs. (D-D”) GFP-Rbpms2bΔRNP1, a deletion of 7 residues within the RRM domain, does not localize to germ granules. (E-F) RFP-Rbpms2b expressed in transgenic Tg[buc:RFP-rbpms2b] ovaries localizes to the Bb (E), while RFP-Rbpms2bΔRNP1 does not (F). (G-J) GFP-Rbpms2b and GFP-Rbpms2bΔRNP1 are stably expressed in gastrula stage embryos (G,H), as well as the granules of HEK293 cells (I, J). (K) GFP immunoprecipitation of Myc- tagged Rbpms2b, Rbpms2bΔRNP1, and Rbpms2bΔC-terminus, a deletion of the last 7 residues within the conserved C-term, demonstrates that the RNP1 domain or conserved C-terminus is not required for interaction with GFP-Bucky ball.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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