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Fig. 2

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ZDB-FIG-220906-2

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Colijn et al., 2022 - High-throughput methodology to identify CRISPR-generated Danio rerio mutants using fragment analysis with unmodified PCR products

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Fig. 2

Fig. 2. cluap1^hi3959a mutants have a 10 kb retroviral insertion in intron 1 of the cluap1 locus and display body axis symmetry defects. (A) Images of control and cluap1^{hi3959a/hi3959a} homozygous mutants at 4 dpf. Mutants display body curvature, a common phenotype of Danio rerio lines with mutant cilia proteins (Cao et al., 2010; Li and Sun, 2011; Sullivan-Brown et al., 2008). (B) Representation of the Danio rerio cluap1 locus. Cluap1 is oriented on the minus strand of chromosome 24 and has 12 exons. The 10 kb retroviral insertion that was used to make the cluap1^hi3959a line sits within intron 1, prior to the translation start site (Li and Sun, 2011).

Expression Data

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Antibody Labeling

Phenotype Data

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Reprinted from Developmental Biology, 484, Colijn, S., Yin, Y., Stratman, A.N., High-throughput methodology to identify CRISPR-generated Danio rerio mutants using fragment analysis with unmodified PCR products, 22-29, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.