FIGURE

Fig. 4

ID
ZDB-FIG-220829-180
Publication
Romagnoli et al., 2022 - Design, Synthesis and Biological Investigation of 2-Anilino Triazolopyrimidines as Tubulin Polymerization Inhibitors with Anticancer Activities
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Fig. 4

(Panel (A)) Quantitative analysis of cell cycle phase distribution in HeLa cells after a 24 h treatment with 3d, at the concentrations of 10, 25 or 50 nM. Cells were stained with PI to analyze the DNA content by flow cytometry. Data are presented as means of three independent experiments ± SEM. (Panel (B)) Effects of compound 3d on cell cycle regulatory proteins and DNA damage checkpoint proteins. HeLa cells were treated for 24 h with the indicated concentration of 3d, and expression of ATR, cdc2 (Y15) and cyclin B was detected by Western blot analysis.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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