Fig. 4

Fig. 4. Genotoxic test shows induction of micronuclei, nuclear anomalies and DNA damage by the KRS-CR Water. A. Micronuclei (MN) and other nuclear abnormalities (NA) (blebbed, reniform, deformed, nucleoplasmic bridge, binucleated and multinucleated observed in treated groups (X, Y and W) zebrafish embryos stained with AO dye at 96 hpf. (Mag. × 60). B. Quantitative analysis of MN and NA observed in zebrafish embryos at 96 hpf. *p < 0.05; p < 0.001. C. DNA damage evaluated by single cell gel electrophoresis (comet assay) (Mag. × 10) in both control and treated groups. D. Quantification of the water samples from Y and Z sites shows significant induction of DNA strand breaks as indicated by comet tail. **p < 0.01; ***p < 0.001; ns: not significant. E. Agarose gel electrophoresis of genomic DNA exposed to the water samples. The genomic DNA of the Y and Z are becoming fragmented, thus fading when compared to the control (Black arrows). a – Normal Nucleus; b – Blebbed Nucleus; c– Reniform Nucleus; d – Deformed Nucleus; e – Micronucleus; f – Nucleoplasmic Bridge; g – Binucleated; h – Multinucleated.