Fig. 2

cfap53^−/− fish are fertile. (A,B) Boxplots for sperm cell analysis showing the total number (horizontal lines) of sperm cells counted (A) and percentage of motile sperm cells (B) in spermatozoa derived from wild type (n=7) and cfap53^−/− males (n=7). Boxes indicate upper and lower quartiles and whiskers indicate upper and lower extremes. (C) Confocal images showing DAPI staining and EdU incorporation in MPcfap53^−/− embryos and lack of EdU incorporation in unfertilized eggs. EdU incubation was performed from 0 to 60 mpf. Quantification of the results are presented in Table 2. (D) Confocal images of one-cell stage embryos stained with DAPI showing either lack of nuclear congression (bottom) or initiation of nuclear congression (top) of male and female pronuclei. Graph shows a quantification of observed nuclear congression in wild-type and MPcfap53^−/− embryos. (E) Graph shows quantification of observed cytokinesis. Numbers of embryos analyzed are indicated in the bars. Embryos were obtained from at least three independent pairs. An unpaired Student's t-test was used to test for significance. Scale bars: 20 μm. Schematics of embryonic development are adapted, with permission, from Kimmel et al. (1995).