FIGURE

Fig. 4

ID

ZDB-FIG-220812-51

Publication

Dong et al., 2022 - VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion

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Fig. 4

Vps28 controlling the secretion of EVs in zebrafish

(A) Confocal images analysis of CD63 positive particles in zebrafish CNS at 54 hpf. Huc:CD63-eGFP plasmids were microinjected into the 1-cell stage zebrafish Vps28 mutants, the area outline by the rectangles upper graph are enlarged in lower graph.

(B) CD63 positive particles were dramatically decreased in vps28 knockout embryos.

(D) Western analysis of EVs marker CD63 expression in 2.5dpf vps28 siblings and vps28 depletion embryos.

(E) TEM analysis of representative fields with MVBs in vps28 siblings and vps28 depletion embryos at 54 hpf. The area outlined by the rectangles in the upper graph is enlarged in the lower graph. Scale bar, 2 μm.

(F) Quantification of MVB numbers in the fields of vps28 siblings and vps28 mutants (n = 6). Data are represented as mean +/− SD. ∗p < 0.05, ∗∗p < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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