FIGURE

Fig. 4

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ZDB-FIG-220805-39

Publication

Makarova et al., 2022 - Benchtop X-band electron paramagnetic resonance detection of melanin and Nitroxyl spin probe in zebrafish

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Fig. 4

Fig. 4. Detection of the eumelanin in zebrafish embryos loaded into the specially-shaped glass capillary made from 4 mm tubes. EPR parameters were modulation amplitude 500 μT, 16 scans, 25 dB, 11s sweet time. Tap panel - integrated CW X-band EPR with eSpect⁺ signal from melanin radical originating from 40 zebrafish larvae. Lower panel - representative bright-field images of 3 days old zebrafish larvae: a) untreated wild-type (WT), b) untreated albino, c) WT treated with 3 ppm PTU, and d) WT treated with 6 ppm PTU. Ventral views of the zebrafish head. Scale bar = 0,5 mm. Standard derivative CW X -band spectra are presented as supplementary materials, Fig. S9.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Free radical biology & medicine, 183, Makarova, K., Zawada, K., Wiweger, M., Benchtop X-band electron paramagnetic resonance detection of melanin and Nitroxyl spin probe in zebrafish, 69-74, Copyright (2022) with permission from Elsevier. Full text @ Free Radic. Biol. Med.