FIGURE

Fig. 7

ID

ZDB-FIG-220721-16

Publication

Batista et al., 2022 - Targeting chalcone binding sites in living Leishmania using a reversible fluorogenic benzochalcone probe

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Fig. 7

Fig. 7. Cytometric quantification and microscopic fluorescence imaging of HAB (5 µM) in live L. amazonensis promastigotes (20,000 cells/µL) in presence of competitors 10-11 (100 µM). Co-markers were 7-AAD (40 µM), LTR (100 nM) or MitoRed (50 nM). A: morphological biparametric plot (FS, cell size; SS, cell granulosity); B: gated plot (FL4: 7-AAD, FL10: HAB); C: 7-AAD cytometric histogram; D: HAB cytometric histogram; E and I: bright field (BF); F and J: HAB labelling; G: LTR labelling; K: MitoRed labelling, H and L: merge. IC₅₀ values of competitors 10-11 are given in Table 1. The results were done in triplicates (cytometric means and standard deviations are given in Table 1) and are representative of three independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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