ZFIN Image: Batista et al., 2022, Fig. 7

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Fig. 7

Fig. 7. Cytometric quantification and microscopic fluorescence imaging of HAB (5 µM) in live L. amazonensis promastigotes (20,000 cells/µL) in presence of competitors 10-11 (100 µM). Co-markers were 7-AAD (40 µM), LTR (100 nM) or MitoRed (50 nM). A: morphological biparametric plot (FS, cell size; SS, cell granulosity); B: gated plot (FL4: 7-AAD, FL10: HAB); C: 7-AAD cytometric histogram; D: HAB cytometric histogram; E and I: bright field (BF); F and J: HAB labelling; G: LTR labelling; K: MitoRed labelling, H and L: merge. IC₅₀ values of competitors 10-11 are given in Table 1. The results were done in triplicates (cytometric means and standard deviations are given in Table 1) and are representative of three independent experiments.

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