FIGURE

Fig. 6

ID

ZDB-FIG-220720-52

Publication

Zasu et al., 2022 - NADPH-Oxidase Derived Hydrogen Peroxide and Irs2b Facilitate Re-oxygenation-Induced Catch-Up Growth in Zebrafish Embryo

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Fig. 6

Irs2b, but not Irs2a, is required for Reoxy induced catch-up growth in the zebrafish embryo via its Reoxy-specific Erk1/2 activation function. (A) Changes in head-trunk angle. Data are average ± SD, n = 7-12. Embryos lacking the irs2b expression (irs2b KD) or control MO injected embryos were used for experiments. (B) Relative growth rates in Norm (26-32.5 hpf) and Reoxy (32-48.5 hpf) group. Data are mean ± SE of 3 independent experiments. Values marked with different letters (a, b) are significantly different from each other (P<0.05), but values marked with common letters (a and ab; ab and b) are not significantly different from each other (P>0.05). N.S. means not significantly different (P>0.05). (C) Immunoblot analysis of the phosphorylation levels of Akt and Erk1/2 under Reoxy condition (48 hpf). Data are mean ± SE of three independent experiments. The asterisk (*) denotes statistical difference at P<0.05. (D) Rescue experiments. Changes in head-trunk angle in the indicated experimental groups. Data are mean ± SE of 2-4 independent experiments. Values marked with different letters (a, b) are significantly different from each other (P<0.05).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)