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Fig. 6
Irs2b, but not Irs2a, is required for Reoxy induced catch-up growth in the zebrafish embryo via its Reoxy-specific Erk1/2 activation function. (A) Changes in head-trunk angle. Data are average ± SD, n = 7-12. Embryos lacking the irs2b expression (irs2b KD) or control MO injected embryos were used for experiments. (B) Relative growth rates in Norm (26-32.5 hpf) and Reoxy (32-48.5 hpf) group. Data are mean ± SE of 3 independent experiments. Values marked with different letters (a, b) are significantly different from each other (P<0.05), but values marked with common letters (a and ab; ab and b) are not significantly different from each other (P>0.05). N.S. means not significantly different (P>0.05). (C) Immunoblot analysis of the phosphorylation levels of Akt and Erk1/2 under Reoxy condition (48 hpf). Data are mean ± SE of three independent experiments. The asterisk (*) denotes statistical difference at P<0.05. (D) Rescue experiments. Changes in head-trunk angle in the indicated experimental groups. Data are mean ± SE of 2-4 independent experiments. Values marked with different letters (a, b) are significantly different from each other (P<0.05).