Fig. 2

TU presents higher expression of genes regulating inflammation including NF-kB and IL-10. Control or larvae exposed to increasing doses of TNBS (T60-T90) were euthanized at 6 dpf, RNA was extracted, and real time PCR was performed. Relative gene expression was calculated using the 2^(-ΔΔCT) method, using the elongation factor 1 - alpha (elf1-α) as internal control. (A) NF-kB expression was higher in TU compared to AB in CTR, T60 and T70, AB vs. TU, *P ≤ 0.05 (B) TNF- α was higher in TU in CTR and T60, AB vs. Tü; *P ≤ 0.05 (C) IL-12 analysis showed no significant differences between AB and TU (D) IL-1β expression significantly increased in TU after high exposure of TNBS (T70); AB vs. TU, *P ≤ 0.05. (E) IL-10 expression was generally more elevated in TU compared to AB in CTR and T70, *P ≤ 0.05, **P ≤ 0.01. (F) IL-1β/IL-10 ratio analysis showed higher ratio of IL-1β in AB compared to TU in control and T90, AB vs TU; *P ≤ 0.05, **P ≤ 0.01; however in T60, ratio was lower compared to AB CTR, **P ≤ 0.01. Each data point corresponds to 2–9 samples, each sample was obtained from a pool of 20 larvae. Data are presented as mean and standard error of mean and differences between AB and TU, or doses were calculated by unpaired t-student test.